PSF Optimization for 3D Single-Molecule Localization Microscopy.

ABOUT THE WEBINAR
In this webinar we will present how the MicAO 3DSR add-on unit can be implemented to any inverted microscopes and how it drastically improves the number and accuracy of localizations in single-molecule localization microscopy (SMLM) and single-particle tracking (SPT) experiments.

We will address the following questions:

What is adaptive optics (AO) and how does it work ?
How can AO be applied to microscopy and SMLM in particular ?
What PSF shaping can you achieve with MicAO ?
Who should attend this webinar?

Researchers and scientists who are working on 2D and/or 3D SMLM (PALM, STORM, PAINT) and SPT experiments, and who need significantly better SNR and/or localization accuracy.

We are looking forward to your attendance.

REGISTER HERE

For your questions please contact Vincent Renaud, vrenaud@64.225.125.189 .

Montage of a 4µm z-stack with 25nm steps of a diffraction limited fluorescent bead with astigmatic PSF shaping achieved with MicAO.

Montage of a 4µm z-stack with 25nm steps of a diffraction limited fluorescent bead with tetrapod PSF shaping achieved with MicAO.

Those montages are two z-stacks of the same fluorescence bead, with the same depth and same number of steps. We clearly see in the montage on the right that the localization of the PSF can be achieved on a much larger range compare to the montage on the left. This is achieved thanks to the tetrapod PSF. On the other hand, both the axial and lateral localization accuracy are better on the left montage, because SNR is higher and PSF variation is sharper.

Join us next Thursday at 12 PM EDT to learn more about how you can achieve both PSF shaping and more!