AO Kit Bio | Adaptive Optics Kit for Microscopy

AO Kit Bio allows you to improve your biological imaging using an adaptive optics loop. Whichever microscopy or OCT method you are using, adaptive optics allows you to pre-compensate aberrations present in your imaging system and to improve your image quality.

AO Kit Bio is an adaptive optics kit for microscopy or OCT, composed of a Shack-Hartmann wavefront sensor (HASO4 First for visible or HASO4 SWIR for the infrared spectral range), Mirao 52e or any other deformable mirror or phase modulator (please contact Imagine Optic in advance to check the compatibility and availability), as well as adaptive optics software. You can choose between the WaveKit Bio set of functions (SDK) or a fully functional adaptive optics software with a user interface. Both software control all functionalities of the wavefront sensor and deformable mirror, can operate these devices in closed and open-loop modes and contain image-based (sensorless) aberration detection algorithms (3N and phase diversity) adapted for microscopy applications.

Available in a variety of hardware configurations for open and closed-loop use, the adaptive optics kit for biology is the solution for researchers who want to incorporate adaptive optics into their custom-built imaging systems. AOKit Bio is compatible with different phase modulators and HASO wavefront sensors. Combining this mirror with the accuracy of HASO wavefront sensors and the ease of use of our adaptive optics software, AOKit Bio is your key to successful imaging.

We provide two different adaptive optics software to control the hardware components of AO Kit Bio package. For easy and fast implementation we recommend using MicAO Soft, which has been specifically designed for aberration detection in microscopy. With a simple user interface, this program controls all the functions of the wavefront sensor and deformable mirror, both in closed and open-loop modes. For aberration detection it is using sensorless, image-based iterative aberration detection methods, genetic and 3N. For implementation of these methods into the home-built software we also provide WaveKit Bio, the Software Development Kit (SDK) of MicAO Soft.

We recommend implementing AO Kit Bio, adaptive optics for microscopy, for the following microscopy techniques: wide field, confocal, multiphoton, light sheet, STED and SIM microscopy, as well as FCS or optical tweezers.