Rescan Confocal Microscopy Webinar

About this Webinar

This webinar is a virtual demo of the Rescan Confocal Microscopy (RCM) unit. The RCM can transform your widefield microscope to a confocal microscope and has all the features and benefits of a regular confocal microscope but additionally offers:

• Improved signal-to-noise ratio (4x)
• Improved lateral resolution (up to 170 nm)
• Low-cost due to its unique camera-based design and flexible system architecture

RCM is a super-resolution technique based on a standard laser-scanning confocal system, extended with an optical re-scanning unit. The re-scanner writes the image directly onto a camera chip. By doubling the sweep of the re-scanning mirrors, the image is magnified on the camera chip without increasing the size of the rescanning spot. This results in an increase in resolution up to 170nm – an improvement of 1.4 times. The camera-based detection and open pinhole design offer a high signal-to-noise ratio, while maintaining confocal sectioning capability. The rescanning principle is optics-mechanics only, achieving an improved resolution image without any post-processing.

Equipment details:

Nikon Ti Eclipse microscope. RCM and Hamamatsu Orca Flash V3 camera will be added on the left port. For excitation the 4-channel Omicron lighthub will be used. This is a 4-channel laser combiner with 100mW output max per channel.

Sample details:
-BPAE cells (Nikon prep), 3-color, DAPI, Actin-488 and Mitotracker red.
-Chromosomes, SYCP3 Alexa488

Webinar Details

Presenters

Jeroen Kole – Product Application Specialist at Confocal.nl 

Vincent Renaud – Microscopy Specialist at Axiom Optics

Nese Didem Temeltas – Sales Support Engineer at Axiom Optics

Date
July 24, 2019

Time
1 PM ET (10 AM PST)

Duration
45 mins

Please reach out to Nese Didem Temeltas, ntemeltas@64.225.125.189 for your questions

REFERENCES (left to right)

[1] Huvec cells stained with DAPI (blue), Actin (green), Tubulin (red) and Clathrin (yellow). Sample courtesy of Nicolas Touret, Department of Biochemistry, University of Alberta.

[2] N1e-115 neuroblastoma cell with CFP-labelled actin. Image by Stan Hilt Confocal.nl)

[3] U2OS cells with phalloidin-Atto643 staining. Sample and image by Andreas Kurz from the Sauer Laboratory at the University of Würzburg.

[4] BPAE cells imaged with a 60x water immersion objective (NA 1.25). Standard sample from Nikon: nucleus (blue), actin (green) and mitochondria (red).

Registration Fee: