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This webinar is a virtual demo of the Rescan Confocal Microscopy (RCM) unit. The RCM can transform your widefield microscope to a confocal microscope and has all the features and benefits of a regular confocal microscope but additionally offers:
RCM is a super-resolution technique based on a standard laser-scanning confocal system, extended with an optical re-scanning unit. The re-scanner writes the image directly onto a camera chip. By doubling the sweep of the re-scanning mirrors, the image is magnified on the camera chip without increasing the size of the rescanning spot. This results in an increase in resolution up to 170nm – an improvement of 1.4 times. The camera-based detection and open pinhole design offer a high signal-to-noise ratio, while maintaining confocal sectioning capability. The rescanning principle is optics-mechanics only, achieving an improved resolution image without any post-processing.
Equipment details:
Nikon Ti Eclipse microscope. RCM and Hamamatsu Orca Flash V3 camera will be added on the left port. For excitation the 4-channel Omicron lighthub will be used. This is a 4-channel laser combiner with 100mW output max per channel.
Sample details:
-BPAE cells (Nikon prep), 3-color, DAPI, Actin-488 and Mitotracker red.
-Chromosomes, SYCP3 Alexa488
Presenters
Jeroen Kole – Product Application Specialist at Confocal.nl
Vincent Renaud – Microscopy Specialist at Axiom Optics
Nese Didem Temeltas – Sales Support Engineer at Axiom Optics
Date
July 24, 2019
Time
1 PM ET (10 AM PST)
Duration
45 mins
Please reach out to Nese Didem Temeltas, ntemeltas@64.225.125.189 for your questions
REFERENCES (left to right)
[1] Huvec cells stained with DAPI (blue), Actin (green), Tubulin (red) and Clathrin (yellow). Sample courtesy of Nicolas Touret, Department of Biochemistry, University of Alberta.
[2] N1e-115 neuroblastoma cell with CFP-labelled actin. Image by Stan Hilt Confocal.nl)
[3] U2OS cells with phalloidin-Atto643 staining. Sample and image by Andreas Kurz from the Sauer Laboratory at the University of Würzburg.
[4] BPAE cells imaged with a 60x water immersion objective (NA 1.25). Standard sample from Nikon: nucleus (blue), actin (green) and mitochondria (red).