New Approaches in Light Microscopy Workshop 2017

November 8th -10th, 2017
Harvard University
Center for Nanoscale Systems and Center for Biological Imaging
Cambridge, MA 02138, USA

New Approaches in Light Microscopy Workshop 2017

Program schedule ( Download the program by clicking on ‘Download’ button at the bottom of the page)

Day 1: November 8th, 2017 : lectures 

  • Fluorescence Lifetime Imaging Microscopy (FLIM)  Session 

-9:15 – 9:45 AM “FRET & FLIM: Quantifying protein interactions in living cells”, Pfr. Richard Day, Professor, Indiana University School Of Medicine

-9:45 – 10:05 AM : “New in camera-based FLIM: two-phase FLIM”, Johan Hertz, Lambert Instruments, Netherlands

  • Coffee Break  10:05 – 10:20 AM 
  • Quantitative Phase Imaging  Session

-10:20 – 10:50 AM : “Label-free imaging thick specimens using GLIM”, Pfr. Gabriel Popescu, University of Illinois Urbana Champaign

-10:50 – 11:20 AM : “Advances in Quantitative Interferometric Microscopy in Blood Disease and Cancer”, Pfr. Peter So, Massachusetts Institute of Technology, Cambridge, MA

  • 3D super resolution Session

-11:20 – 11:50 AM : “Measuring Endosomal pH Beyond the Diffraction Limit”, Doug Richardson,  Ph.D, Harvard Center for Biological Imaging

-11:50 – 12:10 AM: “Easy STED nanoscopy”, Karsten Bahlmann, Ph.D,  Aberrior Instruments

  • Lunch break  12:10 – 1:00 PM
  • 3D super resolution/adaptive optics Session

-1:00 – 1:20 PM: “Adaptive optics for 3D PALM/STORM”, P. Clemenceau, Co-founder, Axiom Optics, Somerville, MA

-1:20 – 1:50 PM : “Adaptive optics improves two-photon super-resolution imaging”, Yicong Wu, Ph.D, NIH, Bethesda , MD

  • 3D volume scanning microscopy Session

-1:50 – 2:20 PM: “Light Sheet Microscopy: Why and How”, Jon Daniels, Ph.D, Applied Scientific Instruments

-2:20 – 2:50 PM: “Reflective imaging improves spatiotemporal resolution in light sheet microscopy”, Yicong Wu, Ph.D, National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD

-2:50 – 3:20 PM: “Sample friendly high speed light sheet imaging”, Cyprian Wozniak, PhaseView SA, Paris, France

  • Micro-spectroscopy Session

-3:10 – 3:40 PM: “Customizable Raman Microspectroscopy for Bacterial Detection”, Pfr. Elizabeth Vargis, Ph.D, Utah State University, Logan, UT

3:40 – 4:00 PM:“Aberration Free Imaging Spectrometers for Microspectroscopy”, Sebastian Remi, Application Scientist, Princeton Instruments, Andover, MA 

4:00 – 4:30 PM: “Enhanced Darkfield Microscopy and Hyperspectral Imaging (EDFM-HSI) for Nanomaterial Identification in Complex Biological and Environmental Matrices”, Pfr. Sara Brenner, MD MPH, SUNY Polytechnic Institute, Albany , NY 

-4:30 – 4:50 PM: “Advancing Nanoscale Research with Enhanced Darkfield Hyperspectral Microscopy”, Samuel M. Lawrence, CEO, CytoViva, Auburn, AL

  • Coffee Break   4:50  – 5:00 PM 
  • Image Analysis and Data Challenges Session

-5:00 – 5:30 PM: “Managing the Microscopy Data Deluge”, Brian Thompson, Ph.D., Regional Sales Engineer, Imaris

-5:30 – 6:00 PM: “Introduction to Principal Components Analysis and Related Methods on Multivariate/Hyperspectral Images”, Barry Wise, PhD, Eigenvector Research 

-6:00 – 6:30 PM: “CellProfiler: biologist-friendly automated pipelines for image analysis and machine learning”, Kyle Karhohs, Minh Doan, Broad Institute, MIT , Cambridge, MA

7:00pm :  Dinner

 

Day 2: November 9th, 2017 : Lab/hands on sessions, demos and training

25-30 registered Lab participants maximum, 3-4 people per group. Lab participants are encouraged to bring samples of interest for observation.

 

Day 3: November 10th, 2017: Lab/hands on sessions

Each equipment station will be available, for participants who are interested to gain additional training on any microscope and understand the technique more intricately.
Participants are encouraged to bring their specimens of interest. Vendors will be present at each station to answer any queries and concerns. 

List of equipment provided by our generous sponsors and partners for laboratory/hands-on sessions:

-Station 1 : Fluorescence Lifetime Imaging (Lambert Instruments) 

New generation LIFA system based on TOGGLE CCD camera manufactured by Lambert Instruments, mounted on a widefield inverted microscope.
For more info: https://www.lambertinstruments.com/toggel#toggel-specifications

-Station  2 : Quantitative Phase Imaging ( Phioptics) 

Spatial Light Interference Microscope & Gradient Light Interference Microscope system manufactured by Phi Optics, mounted on an inverted widefield microscope.
For more info: http://phioptics.com/products/

-Station 3 : Micro-spectroscopy 1 (Princeton Instruments)

Micro-spectroscopy system consisting of an IsoPlane aberration free spectrograph manufactured by Princeton Instruments, mounted on an inverted widefield microscope for measurements such as micro-absorbance, micro-fluorescence or micro-Raman spectroscopy.

http://www.princetoninstruments.com/products/IsoPlane

-Station 4 : Micro-spectroscopy 2 ( Cytoviva)

Enhanced darkfield and hyperspectral imaging and analysis microscopy system manufactured by CytoViva®, mounted on an upright or inverted widefield microscope.
For more info:  https://cytoviva.com

-Station 5 : Adaptive optics ( Imagine Optic )

MICAO 3DSR adaptive optics unit manufactured by Imagine Optic, mounted on an inverted widefield microscope.
For more info: http://www.imagine-optic.com/en/product/micao-3d-sr/0/0/

-Station 6 : 3D super resolution STED (Aberrior Instruments) 

STEDYCON confocal imaging and STED imaging with x/y resolutions of <40nm, manufactured by Abberior-Instruments, mounted on an upright or inverted microscope.
For more info: http://www.abberior-instruments.com/products/compact-line/

Station 7 : Light sheet  microscopy 1 (Phaseview ) 

ALPHA3 light sheet system manufactured by Phaseview, mounted on an upright or inverted widefield microscope.
For more info: www.alphalightsheet.com

Station 8 : Light sheet microscopy 2 (ASI) 

diSPIM dual-view light sheet microscope manufactured by ASI, mounted on an inverted widefield microscope.
For more info: http://dispim.org

Station 9 : Image Analysis 1 ( Eigenvector Research) 

Solo+MIA a comprehensive chemometrics software package from Eigenvector Research, Inc for use with multivariate / hyperspectral images and includes many methods for pattern recognition, classification and chemical imaging.
For more info: http://www.eigenvector.com

Station 10 : Image Analysis 2  (IMARIS) 

IMARIS core scientific software from Biplane that visualizes, analyzes, segments and quantifies 3D and 4D microscopy datasets.
For more info: http://www.bitplane.com/newrelease

Station 11 : Image Analysis 3 (CellProfiler) 

CellProfiler software, biologist-friendly automated pipelines for image analysis and machine learning , http://cellprofiler.org

 

Here’s An Overview of The Past NALM Workshop:

Schedule


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Registration Fee:

Registration is currently open and will end on Nov 1st.

  • 1 day lecture & lunch for harvard affiliates ( students/scientists with a harvard email) : free ( 75 people max )
  • 1 day lecture & lunch for non-harvard affiliates  : $50 USD
  • Full 3 day registration with lectures, hands-on sessions, lunches & dinners : $200 USD

Click on the register button below to access the Eventbrite page.

Register