New Approaches in Light Microscopy 2019

June 4-6, 2019
Harvard University
Center for Nanoscale Systems and Center for Biological Imaging
Cambridge, MA 02138, USA

PROGRAM OVERVIEW

DAY 1 (06/04): LECTURE SERIES (8.45 AM – 1.10 PM)

LOCATION: Jefferson 250, Harvard University

Session 1: FLIM

Mary-Ann Mycek, PhD. (University of Michigan)

Talk title: “Fluorescence Lifetime Imaging Microscopy: Key Features and Applications”

Dan Needleman, PhD. (Harvard University)

Talk title: “Measuring Metabolism with FLIM”

Session 2: 3D Super Resolution/Adaptive Optics

Georgia Squyres (Harvard University)

Talk title: “Z ring assembly is regulated by FtsZ filament binding proteins”

Joerg Bewersdorf, PhD. (Yale University)

Talk title: Live-cell and in vivo STED Microscopy”

Erik Manders, PhD. (Confocal.nl)

Talk title:“Super-resolution confocal microscope for biomedical research; Rescan Confocal Microscopy (RCM)”

Hiroshi Sasaki, PhD. (Harvard University)

Talk title: “Tracing chromosomes at the nanoscale with DNA-PAINT”

DAY 1 (06/04): LAB SESSIONS (2.00 PM – 7.00 PM)

LOCATION: Rotation information will be given during Registration

Lab session with the equipment generously provided by; Lambert Instruments, Imagine Optic, Abberior Instruments, Confocal.nl, Boston Electronics

DAY 2 (06/05): LECTURE SERIES (8.30 AM – 1.15 PM) 

LOCATION: Jefferson 250, Harvard University

Session 1: Light Sheet Microscopy

Talley Lambert, PhD. (Harvard University)

Talk title: An introduction to applications and challenges in light sheet microscopy

Dae Hee Yun (MIT)

Talk title: “Rapid and holistic molecular imaging of large-scale biological systems”

Shoh Asano, PhD. (Pfizer)

Talk title: Expansion Lattice Light-Sheet Microscopy for Rapid Nanoscale Imaging of Whole Brains

Session 2: Image Analysis and Data Challenges

Jennifer Catherine Waters, PhD. (Harvard University)

Talk title: Designing a Rigorous Microscopy Experiment: Image Correction and Validation

Min Guo, PhD. (NIH)

Talk title: “Accelerating iterative deconvolution and multiview fusion by orders of magnitude”

Session 3: Micro-Spectroscopy

Andrew Joe (Harvard University)

Talk title: “Control of Interlayer Excitons in Van der Waals Heterostructures”

Herdeline Ann M. Ardona, PhD. (Harvard University)

Talk title: “Utilizing Integrated Hyperspectral/Raman Darkfield Microscopy as a Label-Free Approach Towards Evaluating the Interactions of Engineered Nanomaterials with Biological Barriers”

DAY 2 (06/05): LAB SESSIONS (2.15 PM – 7.15 PM)

LOCATION: Rotation information will be given during Registration

Lab session with the equipment generously provided by; Montana Instruments, Princeton Insturments, DRVision, ASI, CytoViva, Argolight

DAY 3 (06/06): Bring Your Own Sample Day

LifeCanvas Open House (TBD)

DESCRIPTION

Equipment is provided by our generous sponsors and partners for laboratory/hands-on sessions

Day 1-2: Afternoon Lab/hands-on sessions, demos and training

30 registered Lab participants maximum, 5-6 people per group. Lab participants are encouraged to bring samples of interest for observation.

Day 3: Lab/hands-on sessions

Each equipment station will be available, for participants who are interested to gain additional training on any microscope and understand the technique more intricately.
Participants are encouraged to bring their specimens of interest. Vendors will be present at each station to answer any queries and concerns.

LIST OF STATIONS FOR LAB SESSIONS

Day 1:

–Station 1: Fluorescence Lifetime Imaging (Lambert Instruments)

Toggel is a FLIM camera that simplifies functional imaging for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis. This camera helps minimizing measurement duration, automating image acquisition and simplifying data analysis using LIFA software .It has a frame rate of 30 fps and large pixel size of 24 µm. For more info: https://www.lambertinstruments.com/toggel#toggel-specifications

–Station 2: 3D Super-Resolution STED (Aberrior Instruments)

STEDYCON confocal imaging and STED imaging with x/y resolutions of <40nm, manufactured by Abberior-Instruments, mounted on an upright or inverted microscope.It is a new class of nanoscope and converts your conventional epifluorescence microscope into a powerful multicolor confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system.  For more info: http://www.abberior-instruments.com/products/compact-line

–Station 3: MicAO Adaptive Optics (Imagine Optic)

MicAO 3DSR is a plug and play device that brings the advantages of adaptive optics (AO) to microscopy. It is specifically designed for single molecule localization microscopy (SMLM) methods such as photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM) and single particle tracking (SPT). The high optical transmission of MicAO 3DSR and its ability to correct aberrations enables the detection of more photons from the sample, which increases localization precision of SMLM methods. For more info: http://www.imagine-optic.com/en/product/micao-3d-sr/0/0/

–Station 4:Multi Photon FLIM with TCSPC (Boston Electronics)

The multiphoton FLIM system consists of a Newport DeepSee Insight laser tunable from 680-1300 nm coupled to a Leica SP5 laser scanning confocal microscope.The detector is a HPM-100-40(sensitive from 250-720nm) from Becker and Hickl with a SP-150 Time-Correlated Single Photon Counting (TCSPC) card. TCSPC is a technique low-level light signals with picosecond time resolution.  For more info: https://www.boselec.com/product-category/photon-counting/

–Station 5: Confocal Super Resolution (Confocal.NL)

Confocal.nl Rescan Confocal Microscope (RCM) combines a super-resolution capability with higher sensitivity than most confocal microscopes. It is an affordable upgrade to an existing wide-field fluorescence system. It has a scanning speed up to 4 frames per second. The standard resolution of the RCM is 170 nm at 488 nm wavelength. Deconvolution can be used to improve the RCM resolution even further down to 120 nm. RCM 1.1 has FOV of 160 x 160 μm. For more info: http://www.confocal.nl

Day 2:

–Station 6: Image Analysis (Argolight/Argo-Power)

Argo–POWER is a device designed to quickly assess the quality of your microscope. Power measurement, resolution, 3D, a solution for your troubleshooting. Argo-HM slide has stable fluorescents patterns and its compatible with air, water and oil immersion leases. The operating wavelength ranges form 350 nm to 650nm. The Power meter on the device can measure power from 10 µW to 100 mW with an operating wavelength range of 350 nm to 1100 nm and it allows measuring in real time. For more info: http://argolight.com/products/argo-power/

–Station 7: Light Sheet Microscopy (Applied Scientific Instrumentation)

diSPIM dual-view light sheet microscope manufactured by Applied Scientific Instrumentation mounted on an inverted widefield microscope. It has an acquisition rate up to 200 planes per second and has low photobleaching, >10x reduction vs. confocal/ spinning disk. The imaging depth is limited by scattering, usually 30 -150 µm depending on sample.  For more info: http://dispim.org

–Station 8:Hyperspectral Microscope (CytoViva)

The Cytoviva Darkfield Hyperspectral microscope can provide spectral data from 420-1000nm for every pixel in the field of view. The darkfield hyperspectral data allows for spectral signatures of the different types of nanoparticles to be collected. It is mounted on a Horiba Xplora confocal Raman microscope with a 405 and 785 nm excitation lasers, so correlative Raman and hyperspectral data can be collected.  For more info:
https://cytoviva.com/products/hyperspectral-imaging-2/hyperspectral-imaging/

–Station 9:Cyro-Raman (Montana Instruments and Teledyne Princeton Instruments)

Many interesting phenomena emerge at cryogenic temperatures, and often, additional or new information about a sample can be obtained with temperature-dependent measurements. The CryoRaman variable temperature Raman microscope system is optimized for high collection efficiency and throughput, offering an automated and controlled environment for characterizing materials with standard spectroscopic techniques. The system allows for rapid, user-defined temperature control between 4K-500K as well as creation of Raman maps of samples and gives access to simultaneous electrical measurements as well. For more info https://www.montanainstruments.com/Products/CryoRaman

– Station 10:  Aivia (Drvision)

Based on 18 years of in-house R&D, Aivia is an innovative and complete 2-to-5D image visualization and analysis platform. Using state-of-the-art algorithm and software architecture, Aivia delivers top performance on critical tasks such as display of large images and analysis of complex biological phenomena. Aivia is powered by a range of AI technology for image segmentation, deconvolution and virtual staining. For more info:https://www.drvtechnologies.com

Day 3:

–Station 10: Light Sheet Microscopy (LifeCanvas)

SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed and resolution. The system was designed from the ground up, with no limitation based on commercially available microscope bodies. It has a frame rate of 20 FPS during z-stack acquisition. The axial resolution of the device with 3.6x objective, NA 0.2, PSFz < 4.5 µm and FOV of 3700 µm. For more info: https://lifecanvastech.com/smartspim/

**All the stations will be available on Day 3. Registration for stations will be open during the workshop please register for available time slots.

Registration Fee:

Registration is now open! Please click the download button for the FINAL program overview

  • Half day lecture & lunch for Harvard affiliates (students/scientists with a Harvard email): free (max 70 people/day)
  • Half day lecture & lunch for non-Harvard affiliates: $25 USD/day
  • Full 3-day registration with lectures, hands-on sessions, lunches & dinners: $300 USD
  • Full 3-day registration with lectures, hands-on sessions, lunches & dinners for Harvard affiliates: $250 USD
Register