About this Webinar
This webinar is a virtual demo of the Rescan Confocal Microscopy (RCM) unit. The RCM can transform your widefield microscope to a confocal microscope and has all the features and benefits of a regular confocal microscope but additionally offers:
- Improved signal-to-noise ratio (4x)
- Improved lateral resolution (up to 170 nm)
- Low-cost due to its unique camera-based design and flexible system architecture
RCM is a super-resolution technique based on a standard laser-scanning confocal system, extended with an optical re-scanning unit. The re-scanner writes the image directly onto a camera chip. By doubling the sweep of the re-scanning mirrors, the image is magnified on the camera chip without increasing the size of the rescanning spot. This results in an increase in resolution up to 170nm – an improvement of 1.4 times. The camera-based detection and open pinhole design offer a high signal-to-noise ratio, while maintaining confocal sectioning capability. The rescanning principle is optics-mechanics only, achieving an improved resolution image without any post-processing.
Nikon Ti Eclipse microscope. RCM and Hamamatsu Orca Flash V3 camera will be added on the left port. For excitation the 4-channel Omicron lighthub will be used. This is a 4-channel laser combiner with 100mW output max per channel.
-BPAE cells (Nikon prep), 3-color, DAPI, Actin-488 and Mitotracker red.
-Chromosomes, SYCP3 Alexa488
Jeroen Kole – Product Application Specialist at Confocal.nl
Vincent Renaud – Microscopy Specialist at Axiom Optics
Nese Didem Temeltas – Sales Support Engineer at Axiom Optics
July 24, 2019
1 PM ET (10 AM PST)
Please reach out to Nese Didem Temeltas, email@example.com for your questions
REFERENCES (left to right)
 Huvec cells stained with DAPI (blue), Actin (green), Tubulin (red) and Clathrin (yellow). Sample courtesy of Nicolas Touret, Department of Biochemistry, University of Alberta.
 N1e-115 neuroblastoma cell with CFP-labelled actin. Image by Stan Hilt Confocal.nl)
 U2OS cells with phalloidin-Atto643 staining. Sample and image by Andreas Kurz from the Sauer Laboratory at the University of Würzburg.
 BPAE cells imaged with a 60x water immersion objective (NA 1.25). Standard sample from Nikon: nucleus (blue), actin (green) and mitochondria (red).