NL5 (Confocal Microscope / Add-on)

NL5 (Confocal Microscope / Add-on)

Modality

Laser-Scanning Confocal Microscopy

Configuration

Widefield & Confocal / Upright or Inverted

Resolution

170 nm (after deconvolution, raw image = 240 nm)

Sensitivity

Up to 95% QE

FOV

330 x 330 µm (40x magnification)

Speed

25 FPS

New Line. New Light. New Live.

What is NL5?

NL5 is a fast line-scanning confocal system with high sensitivity and resolution. It allows you to quickly screen a multi-well plate with multicolor images, from which you can select the most promising ones. Capture 3D live samples with minimal phototoxicity, high resolution and contrast. Like RCM, NL5 keeps your samples happy under the microscope for a long time and is easy to use. Convert your research microscope into a fast-scanning confocal microscope that provides very gentle conditions for your live samples.

Contrary to spinning-disk systems, another advantage of NL5 and its slit pinhole is that it does not lead to pinhole crosstalk when imaging deep within the sample. NL5 reaches 240 nm lateral resolution which can be easily converted into 170 nm with on the fly deconvolution.

NL5 is an excellent add-on to any research microscope. It allows for converting a wide-field system into a fast scanning confocal with high resolution and sensitivity.

Fast scanning speed, low levels of phototoxicity

 

In the field of biology, some natural processes happen fast, like cell and protein dynamics and cell-cell interactions. Therefore, fast scanning speed is needed to study them. And that is exactly what NL5 brings to the table! In addition, researchers in developmental biology, regeneration or immunology fields often need to image large live samples (mouse embryos, zebrafish, organoids) for a long time without losing any detail of the cell’s behavior. In that case, a combination of speed and a low phototoxicity system like NL5 is perfect.

The benefits of using NL5

NL5 is a fast scanning confocal system that reaches 25 fps (full frame), much faster than regular point-scanning confocal systems. In addition to speed, it brings very gentle imaging conditions for your samples as well. Perform live cell imaging for more than a day, with no signs of phototoxicity. NL5 is easy to use, and requires basic training to operate.

NL5 does not cause any pinhole cross-talk, as it has only one slit pinhole. Pinhole cross-talk is associated with spinning disk systems, because they contain a disk with multiple pinholes combined and the out-of-focus light can sometimes pass through the closeby pinholes. This effect is more evident when imaging deep within the sample, making NL5 the ideal system for those applications.

NL5 is a cost-effective solution that can be coupled to any research microscope via the c-mount interface

Who should use NL5 and why?

High resolution & low phototoxicity

NL5 is an easy-to-use and compact confocal microscope system, a great addition to any microscopy facility. It’s a fruitful solution for individual research labs to easily convert any existing widefield fluorescence microscope into a fast confocal microscope system with high sensitivity and resolution. Capture datasets of 170nm lateral resolution after deconvolution at 25 fps. Study fast cell and molecular dynamics, or perform high contrast deep 3D imaging with minimal aberrations. The high sensitivity of the modern sCMOS cameras used as detectors facilitates acquisition with low light sample exposure, making NL5 ideal for live cell imaging

Convert your existing research microscope into a fast-scanning confocal system ideal for deep 3D live cell imaging.

  • Single digital scanner (same as RCM2)
  • 170 nm resolution in real time (microvolution)
  • Extreme sensitivity
  • Low phototoxicity
  • NL5 runs at a speed of 25 fps
  • High contrast deep 3D imaging without pinhole cross-talk
  • High sensitivity and resolution (170 nm after deconvolution)
  • Live cell friendly
  • Hardware integration into third-party software
  • Compact & easy to use

*Videos above:

(1) Max projection of a stack in time with 1000 frames per color HUVEC labeled for SPY505-DNA, SPY555-Actin and SPY650-Tubulin Sample courtesy of Philippa Phelp (Boon’s Lab) Physiology department. VUmc Amsterdam

(2) Zebrafish embryo (24 to 48 hpf). Endothelial cells labelled with GFP Sample courtesy: Kinki Jim. VUmc Amsterdam, MMIC

(3) HeLa Cell with cytosolic GFP. Sample courtesy of Caroline Pham (Lodder’s Group). Experimental Cardiology Department. VUmc location AMC, Amsterdam