AO Kit Bio | Adaptive Optics Kit for Microscopy
Characterize Time
Takes less than 30 minutes
Compatibility
With Low (LM) & High (HM) lenses
Compatibility
With Low (LM) & High (HM) lenses
Performances
Fluorescent-based imaging systems
Performances
Fluorescent-based imaging systems
Characterize Time
Takes less than 30 minutes
AO Kit Bio Adaptive Optics Kit
AO Kit Bio allows you to build the adaptive optics kit that best suits your individual needs. Whichever microscopy method you are using, adaptive optics allows you to pre-compensate aberrations present in your imaging system and to improve your image quality.
AO Kit Bio is a set of adaptive optics kit components, composed of a Shack-Hartmann wavefront sensor (HASO4 First for visible or HASO4 SWIR for the infrared spectral range), Mirao 52e or any other deformable mirror or phase modulator (please contact Imagine Optic in advance to check the compatibility and availability), as well as adaptive optics software. You can choose between the WaveKit Bio set of functions (SDK) or a fully functional adaptive optics software with a user interface. Both software control all functionalities of the wavefront sensor and deformable mirror, can operate these devices in closed and open-loop modes and contain image-based (sensorless) aberration detection algorithms (3N and phase diversity) adapted for microscopy applications such as confocal imaging.
We recommend implementing the AO Kit Bio adaptive optics kit for the following microscopy techniques: wide field, confocal microscopy, multiphoton, light sheet, STED and SIM microscopy, as well as FCS or optical tweezers.
| DESCRIPTION | SPECIFICATION |
|---|---|
| Number of actuators | 52 |
| Maximum generated wavefront (PV) | ±50 µm |
| Surface quality (rms active flat) | 10 nm |
| Integrated tip/tilt correction | yes |
| Spatial frequency correction Zernike orders | up to 6 |
| Effective diameter | 15 mm |
| Linearity | >95% |
| Hysteresis | <2% |
| Actuator input voltage | ±1V maximum* |
| Coating | protected silver |
| Power consumption | 50W max |
| Dimensions / weight | 64 x 64 x 23 mm3 / 490g** |
| Connectivity | USB 2.0 |
| Operating System | Windows XP, Windows 7 (32 and 64 bit) and Windows 10 |
| HASO4 FIRST | |
|---|---|
| Aperture dimension | 3.6 x 4.6 mm² |
| Number of microlenses | 32 x 40 |
| Tilt dynamic range | > ± 3 ° (400 λ) |
| Focus dynamic range | ± 0.018 m to ± ∞ (350 λ) |
| Focus dynamic range – maximum NA | 0.1 |
| Repeatability (rms) | < λ/200 |
| Wavefront measurement accuracy in relative mode (rms)* | ~ λ/150 |
| Wavefront measurement accuracy in absolute mode (rms)** | ~ λ/100 |
| Tilt measurement sensitivity (rms) | 5 μrad |
| Focus measurement sensitivity (rms) | 3·10-3m-1 |
| Spatial resolution | ~ 110 μm |
| Maximum acquisition frequency | 100 Hz |
| Processing frequency (CPU 3Ghz, 512 Mb RAM) | 20 Hz |
| Working wavelength range | 350 – 1100 nm |
| Calibrated wavelength | in the 400-1100 nm range, details on request*** |
| Extended wavelength range | not available |
| Dimensions / weight | 42x 42 x 42 mm3 /150g |
| Working temperature | 15 – 30° C |
| Interface / Power supply | USB 3.0 / 2.7 W via USB |
| Operating system | Windows 7 (32 and 64 bit) and Windows 10 |
| WaveViewTM metrology software | included |
| External trigger | yes |
AO Kit BIO
The AO Kit Bio consists of the HASO4 First wavefront sensor and the Mirao 52e electromagnetically deformable mirror.
- PALM/STORM and SPT
- Multiphoton microscopy
- SPIM or Light Sheet Microscopy
- STED microscopy
- Confocal and spinning disk microscopy
Microscopy & ImagingÂ
- Deep 3D PALM/STORM Imaging: MicAO 3DSR – The key to combining depth and highest resolution
- 3D Dual-color PALM/dSTORM imaging of centrosomal proteins with nanometric resolution using MicAO 3DSR
- Temporal Stabilization Module Inside MicAO 3DSR
- Simple Method to Improve Image Contrast in Spinning Disk Microscopy Using Adaptive OpticsÂ
- Quasi-isotropic Nanometric 3D Resolution in PALM/STORM with the help of MicAO 3DSR